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Electrochemical aptasensor for lung cancer-related protein detection in crude blood plasma samples / G. S. Zamay [et al.] // Sci. Rep. - 2016. - Vol. 6. - Ст. 34350, DOI 10.1038/srep34350. - Cited References:24. - Authors thank Dr. Mahmoud Labib and Galina Kudryasheva. This work was supported by Ministry of Education and Science of Russian Federation Federal Target Program #14.604.21.0105 for Anna S. Zamay. Electron microscopy was carried out at the Multiple-Access Center of Krasnoyarsk Research Center Siberian branch of Russian Academy of Science. . - ISSN 2045-2322

РУБ Multidisciplinary Sciences
Рубрики:
SQUARE-WAVE VOLTAMMETRY
   GENE-EXPRESSION
   SERUM
   BIOSENSORS
   DIAGNOSIS
   ADENOCARCINOMA
   BIOMARKERS
   SENSOR
Аннотация: The development of an aptamer-based electrochemical sensor for lung cancer detection is presented in this work. A highly specific DNA-aptamer, LC-18, selected to postoperative lung cancer tissues was immobilized onto a gold microelectrode and electrochemical measurements were performed in a solution containing the redox marker ferrocyanide/ferricyanide. The aptamer protein targets were harvested from blood plasma of lung cancer patients by using streptavidin paramagnetic beads and square wave voltammetry of the samples was performed at various concentrations. In order to enhance the sensitivity of the aptasensor, silica-coated iron oxide magnetic beads grafted with hydrophobic C8 and C4 alkyl groups were used in a sandwich detection approach. Addition of hydrophobic beads increased the detection limit by 100 times. The detection limit of the LC-18 aptasensor was enhanced by the beads to 0.023 ng/mL. The formation of the aptamer -protein -bead sandwich on the electrode surface was visualized by electron microcopy. As a result, the electrochemical aptasensor was able to detect cancer-related targets in crude blood plasma of lung cancer patients.

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Держатели документа:
Krasnoyarsk State Med Univ, Lab Biomol & Med Technol, 1 P Zheleznyaka, Krasnoyarsk 660022, Russia.
Russian Acad Sci, Siberian Branch, Inst Chem & Chem Technol, 50-24 Akademgorodok, Krasnoyarsk 660036, Russia.
Design Dept Iskra, 1 Televizornaya, Krasnoyarsk 660028, Russia.
Russian Acad Sci, Siberian Branch, Krasnoyarsk Res Ctr, 50 Akademgorodok, Krasnoyarsk 660036, Russia.
Krasnoyarsk Reg Clin Canc Ctr, 1 Smolenskaya, Krasnoyarsk 660022, Russia.
Univ Ottawa, Dept Chem, 10 Marie Curie,DIorio Hall,Room 201, Ottawa, ON K1N 6N5, Canada.
Russian Acad Sci, Siberian Branch, Inst Phys, 50-38 Akademgorodok, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Zamay, G. S.; Zamay, T. N.; Kolovskii, V. A.; Shabanov, A. V.; Шабанов, Александр Васильевич; Glazyrin, Y. E.; Veprintsev, D. V.; Krat, A. V.; Zamay, S. S.; Kolovskaya, O. S.; Gargaun, A.; Sokolov, A. E.; Соколов, Алексей Эдуардович; Modestov, A. A.; Artyukhov, I. P.; Chesnokov, N. V.; Petrova, M. M.; Berezovski, M. V.; Zamay, A. S.; Ministry of Education and Science of Russian Federation Federal Target Program [14.604.21.0105]
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The Ca2+-regulated photoprotein obelin as a tool for SELEX monitoring and DNA aptamer affinity evaluation / V. V. Krasitskaya, N. S. Goncharova, V. V. Biriukov [et al.] // Photochem. Photobiol. - 2020. - Vol. 96, Is. 5. - P. 1041-1046, DOI 10.1111/php.13274. - Cited References: 25. - This work has been supported by the Russian Foundation for Basic Research (RFBR) under the grant no 18-38-00531. . - ISSN 0031-8655. - ISSN 1751-1097

РУБ Biochemistry & Molecular Biology + Biophysics
Рубрики:
CARDIAC TROPONIN-I
   BIOLUMINESCENCE
   IMMUNOASSAY
   APTASENSOR
   DIAGNOSIS
Аннотация: Bioluminescent solid-phase analysis was proposed to monitor the selection process and to determine binding characteristics of the aptamer-target complexes during design and development of the specific aptamers. The assay involves Ca2+-regulated photoprotein obelin as a simple, sensitive and fast reporter. Applicability and the prospects of the approach were exemplified by identification of DNA aptamers to cardiac troponin I, a highly specific early biomarker for acute myocardial infarction. Two structurally different aptamers specific to various epitopes of troponin I were obtained and then tested in a model bioluminescent assay.

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Держатели документа:
Fed Res Ctr KSC SB RAS, Inst Biophys SB RAS, Krasnoyarsk, Russia.
Siberian Fed Univ, Krasnoyarsk, Russia.
Inst Chem Biol & Fundamental Med SB RAS, Novosibirsk, Russia.
Fed Res Ctr KSC SB RAS, Kirensky Inst Phys, Krasnoyarsk, Russia.

Доп.точки доступа:
Krasitskaya, V. V.; Goncharova, N. S.; Biriukov, V. V.; Bashmakova, E. E.; Kabilov, M. R.; Baykov, I. K.; Sokolov, A. Е.; Соколов, Алексей Эдуардович; Frank, L. A.; Russian Foundation for Basic Research (RFBR)Russian Foundation for Basic Research (RFBR) [18-38-00531]
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