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Bondar', V. S.
Nanodiamonds for biological investigations / V. S. Bondar', A. P. Puzyr' // Phys. Solid State. - 2004. - Vol. 46, Is. 4. - P. 716-719, DOI 10.1134/1.1711457. - Cited References: 9 . - ISSN 1063-7834

РУБ Physics, Condensed Matter

Аннотация: Nanoparticles with a modified surface are prepared from nanodiamonds produced in Russia. The properties of modified nanodiamonds and their hydrosols and organosols are investigated by biophysicists with the aim of preparing nanoparticles with controlled properties for solving biological problems. (C) 2004 MAIK "Nauka / Interperiodica".

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Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Div, Krasnoyarsk 660036, Russia
ИБФ СО РАН
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Puzyr', A. P.
}
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Bondar', V. S.
Applications of nanodiamonds for separation and purification of proteins / V. S. Bondar', I. O. Pozdnyakova, A. P. Puzyr' // Phys. Solid State. - 2004. - Vol. 46, Is. 4. - P. 758-760, DOI 10.1134/1.1711468. - Cited References: 11 . - ISSN 1063-7834

РУБ Physics, Condensed Matter
Рубрики:
ESCHERICHIA-COLI
OBELIN
Аннотация: Recombinant apoobelin and recombinant luciferase are separated from bacterial cells of Escherichia coli with the use of detonation nanodiamonds. The application of nanodiamonds has a number of points in its favor, namely, (i) simplifies the procedures for purifying the proteins, (ii) decreases the time of their separation to 30-40 min, (iii) eliminates the necessity of using special chromatographic equipment, and (iv) makes it possible to prepare high-purity apoobelin and luciferase materials with protein yields of 35-45 and 45-60%, respectively. The possible mechanisms of interaction of protein molecules and nanodiamond particles are analyzed. (C) 2004 MAIK "Nauka / Interperiodica".

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Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Div, Krasnoyarsk 660036, Russia
ИБФ СО РАН
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Pozdnyakova, I. O.; Puzyr', A. P.
}
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Puzyr', A. P.
Design of a luminescent biochip with nanodiamonds and bacterial luciferase / A. P. Puzyr', I. O. Pozdnyakova, V. S. Bondar' // Phys. Solid State. - 2004. - Vol. 46, Is. 4. - P. 761-763, DOI 10.1134/1.1711469. - Cited References: 10 . - ISSN 1063-7834

РУБ Physics, Condensed Matter

Аннотация: An "aluminum oxide film-adhesive layer-nanodiamond-luciferase" supramolecular structure is prepared on a flat plate. It is demonstrated that, in this structure, the enzyme retains the catalytic activity. The structure prepared can be treated as a luminescent biochip prototype for use in bioluminescent analysis. (C) 2004 MAIK "Nauka / Interperiodica".

WOS,
Scopus,
Читать в сети ИФ
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Div, Krasnoyarsk 660036, Russia
ИБФ СО РАН
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Pozdnyakova, I. O.; Bondar', V. S.
}
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