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Adaptation of a bacterial bioluminescent assay to monitor bioeffects of gold nanoparticles / M. R. Yehia, T. E. Smolyarova, A. V. Shabanov [et al.] // Bioengineering. - 2022. - Vol. 9, Is. 2. - Ст. 61, DOI 10.3390/bioengineering9020061. - Cited References: 91. - This research was funded by RFBR, N18-29-19003; RFBR, Krasnoyarsk Territory and Krasnoyarsk Regional Fund of Science, N20-44-243001; and partly supported by the Program of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Fundamental Study 2020–2025 (Russian Federation) . - ISSN 2306-5354

РУБ Biotechnology & Applied Microbiology + Engineering, Biomedical

Кл.слова (ненормированные):
gold nanoparticles -- luminous marine bacteria -- bioassay -- hormesis -- tritium -- reactive oxygen species
Аннотация: Our current study aimed to adapt a bioluminescent bacteria-based bioassay to monitor the bioeffects of gold nanoparticles (AuNPs). Luminous marine bacteria Photobacterium phosphoreum and AuNPs modified with polyvinylpyrrolidone were employed; low-concentration (≤10−3 g/L) bioeffects of AuNPs were studied. Bioluminescence intensity was used as an indicator of physiological activity in bacteria. Two additional methods were used: reactive oxygen species (ROS) content was estimated with a chemiluminescent luminol method, and bacterial size was monitored using electron microscopy. The bacterial bioluminescent response to AuNPs corresponded to the “hormesis” model and involved time-dependent bioluminescence activation, as well as a pronounced increase in the number of enlarged bacteria. We found negative correlations between the time courses of bioluminescence and the ROS content in bacterial suspensions, demonstrating the relationship between bioluminescence activation and bacterial ROS consumption. The combined effects of AuNPs and a beta-emitting radionuclide, tritium, revealed suppression of bacterial bioluminescent activity (as compared to their individual effects) and a reduced percentage of enlarged bacteria. Therefore, we demonstrated that our bacteria-based bioluminescence assay is an appropriate tool to study the bioeffects of AuNPs; the bioeffects can be further classified within a unified framework for rapid bioassessment.

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Держатели документа:
Siberian Fed Univ, Biophys Dept, Krasnoyarsk 660041, Russia.
RAS, Inst Phys SB, Fed Res Ctr, Krasnoyarsk Sci Ctr SB RAS, Krasnoyarsk 660036, Russia.
RAS, Inst Biophys SB, Fed Res Ctr, Krasnoyarsk Sci Ctr SB RAS, Krasnoyarsk 660036, Russia.
Moscow MV Lomonosov State Univ, Dept Chem, Moscow 119991, Russia.

Доп.точки доступа:
Yehia, M. R.; Smolyarova, T. E.; Смолярова, Татьяна Евгеньевна; Shabanov, A. V.; Шабанов, Александр Васильевич; Sushko, E. S.; Сушко, Екатерина Сергеевна; Badun, G. A.; Kudryasheva, N. S.
}
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    Исследование выноса трития водотоками с территории Красноярского ГХК / А. В. Носов, А. М. Мартынова, В. Ф. Шабанов [и др.] // Атомная энергия. - 2001. - Т. 90, № 1. - С. 77-80
   Перевод заглавия: Studying the tritium removal with water courses from the territory of the Krasnoyarsk GKhK

Доп.точки доступа:
Носов, А. В.; Мартынова, А. М.; Шабанов, Василий Филиппович; Shabanov, V. F.; Савицкий, Ю. В.; Шишлов, Е. А.; Ревенко, Ю. А.
}
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Radioprotective properties of fullerenol: Cellular, biochemical and physicochemical approaches / O. V. Kolesnik, A. S. Grabovoy, G. A. Badun [et al.] // Eurasian J. Chem. - 2024. - Vol. 29, Is. 3 (115), Section : Nano- and photo- catalysis in current chemistry: Possibilities and challenges. - P. 72-81, DOI 10.31489/2959-0663/3-24-9. - Cited References: 65. - This research was funded by the Russian Science Foundation No. 23-26-10018, Krasnoyarsk Regional Science Foundation. The purification of enzymes was supported by the State Assignment of the Ministry of Science and Higher Education of the Russian Federation, project No. FWES-2024-0018 . - ISSN 2959-0663. - ISSN 2959-0671
Кл.слова (ненормированные):
tritium -- fullerenol -- radioprotection -- luminescence -- bacteria -- bioassay -- reactive oxygen species -- enzymes
Аннотация: The search for optimal radioprotective methods and tools under low-dose radiation exposures represents a pressing issue in the field of modern radioecology. The objective of the study was to investigate the radioprotective properties of fullerenol C60,70Oy(OH)x, (x+y = 24–28), a water-soluble polyhydroxylated fullerene derivative with an electron-deficient aromatic carbon structure. Tritium, a radionuclide of low decay energy, was selected to simulate an exposure to low-dose irradiation (˂ 0.05 Gy). We applied luminous marine bacteria Photobacterium phosporeum as a model cellular object to monitor radiation bioeffects; the bioluminescence intensity of the bacteria was used as a tested biological parameter. Tritium activated the bacterial luminescence; the addition of fullerenol (˂ 3∙10–3 g/L) “mitigated” the activation, thus revealing the radioprotective capacity of fullerenol for the marine microorganism. To evaluate the mechanisms of radioprotection of fullerenol in tritiated water, we investigated the effects of fullerenol on: (1) the content of reactive oxygen species and (2) the intensity of bioluminescence in the bacterial enzymatic reaction. Tritiated water produced moderate deviations from the control values, whereas the addition of fullerene brought these values closer to the ‘control’ ones. All observed effects were attributed to variations in the ionic balance of the aqueous medium, which resulted in the activation of bacterial functions through cell membranes.

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Держатели документа:
Federal Research Center "Krasnoyarsk Science Center" of the Siberian Branch of the Russian Academy of Sciences, "Institute of Biophysics of the Siberian Branch of the Russian Academy of Sciences", Krasnoyarsk, Russia
Siberian Federal University, Krasnoyarsk, Russia
Lomonosov Moscow State University, Moscow, Russia
Federal Research Center "Krasnoyarsk Science Center" of the Siberian Branch of the Russian Academy of Sciences, "Institute of Physics of the Siberian Branch of the Russian Academy of Sciences", Krasnoyarsk, Russia

Доп.точки доступа:
Kolesnik, O. V.; Grabovoy, A. S.; Badun, G. A.; Churilov, G. N.; Чурилов, Григорий Николаевич; Kudryasheva, N. S.
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Investigation of the tritium transport by water flows from the territory of the mining-chemical combine in Krasnoyarsk / A. V. Nosov, A. M. Martynova, V. F. Shabanov [et al.] // Atomic energy. - 2001. - Vol. 90, Is. 1. - P. 91-94

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Держатели документа:
Krasnoyarsk Science Center, Siberian Branch of the Russian Academy of Sciences
Krasnoyarsk Mining-Chemical Combine
State Institute of Applied Ecology

Доп.точки доступа:
Nosov, A. V.; Martynova, A. M.; Shabanov, V. F.; Шабанов, Василий Филиппович; Savitskii, Yu. V.; Shishlov, A. E.; Revenko, Yu. A.
}
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