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Bondar', V. S. Applications of nanodiamonds for separation and purification of proteins / V. S. Bondar', I. O. Pozdnyakova, A. P. Puzyr'> // Phys. Solid State. - 2004. - Vol. 46, Is. 4. - P. 758-760, DOI 10.1134/1.1711468. - Cited References: 11
. - ISSN 1063-7834РУБ Physics, Condensed Matter Рубрики: ESCHERICHIA-COLI OBELIN Аннотация: Recombinant apoobelin and recombinant luciferase are separated from bacterial cells of Escherichia coli with the use of detonation nanodiamonds. The application of nanodiamonds has a number of points in its favor, namely, (i) simplifies the procedures for purifying the proteins, (ii) decreases the time of their separation to 30-40 min, (iii) eliminates the necessity of using special chromatographic equipment, and (iv) makes it possible to prepare high-purity apoobelin and luciferase materials with protein yields of 35-45 and 45-60%, respectively. The possible mechanisms of interaction of protein molecules and nanodiamond particles are analyzed. (C) 2004 MAIK "Nauka / Interperiodica".
WOS, Scopus, Читать в сети ИФ Держатели документа: Russian Acad Sci, Inst Biophys, Siberian Div, Krasnoyarsk 660036, Russia ИБФ СО РАН Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation Доп.точки доступа: Pozdnyakova, I. O.; Puzyr', A. P. } Найти похожие
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