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The Ca2+-regulated photoprotein obelin as a tool for SELEX monitoring and DNA aptamer affinity evaluation / V. V. Krasitskaya, N. S. Goncharova, V. V. Biriukov [et al.]> // Photochem. Photobiol. - 2020. - Vol. 96, Is. 5. - P. 1041-1046, DOI 10.1111/php.13274. - Cited References: 25. - This work has been supported by the Russian Foundation for Basic Research (RFBR) under the grant no 18-38-00531.
. - ISSN 0031-8655. - ISSN 1751-1097РУБ Biochemistry & Molecular Biology + Biophysics Рубрики: CARDIAC TROPONIN-I BIOLUMINESCENCE IMMUNOASSAY APTASENSOR DIAGNOSIS Аннотация: Bioluminescent solid-phase analysis was proposed to monitor the selection process and to determine binding characteristics of the aptamer-target complexes during design and development of the specific aptamers. The assay involves Ca2+-regulated photoprotein obelin as a simple, sensitive and fast reporter. Applicability and the prospects of the approach were exemplified by identification of DNA aptamers to cardiac troponin I, a highly specific early biomarker for acute myocardial infarction. Two structurally different aptamers specific to various epitopes of troponin I were obtained and then tested in a model bioluminescent assay.
Смотреть статью, Scopus, WOS, Читать в сети ИФ Держатели документа: Fed Res Ctr KSC SB RAS, Inst Biophys SB RAS, Krasnoyarsk, Russia. Siberian Fed Univ, Krasnoyarsk, Russia. Inst Chem Biol & Fundamental Med SB RAS, Novosibirsk, Russia. Fed Res Ctr KSC SB RAS, Kirensky Inst Phys, Krasnoyarsk, Russia. Доп.точки доступа: Krasitskaya, V. V.; Goncharova, N. S.; Biriukov, V. V.; Bashmakova, E. E.; Kabilov, M. R.; Baykov, I. K.; Sokolov, A. Е.; Соколов, Алексей Эдуардович; Frank, L. A.; Russian Foundation for Basic Research (RFBR)Russian Foundation for Basic Research (RFBR) [18-38-00531] } Найти похожие
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