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Corrigendum to "Properties of degradable polyhydroxyalkanoates with different monomer compositions" International Journal of Biological Macromolecules; Volume 182, Pages 98–114 / T. Volova, E. Kiselev, I. Nemtsev [et al.] // Int. J. Biol. Macromol. - 2024. - Vol. 263, Pt. 1. - Ст. 130252, DOI 10.1016/j.ijbiomac.2024.130252. - Cited References: 1 . - ISSN 0141-8130. - ISSN 1879-0003

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Держатели документа:
Siberian Federal University, 79 Svobodnyi av., Krasnoyarsk 660041, Russia
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, Krasnoyarsk 660036, Russia
Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50 Akademgorodok, Krasnoyarsk 660036, Russia
L.V. Kirensky Institute of Physics, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/12 Akademgorodok, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Volova, T.; Kiselev, E.; Nemtsev, I. V.; Немцев, Иван Васильевич; Lukyanenko, A. V.; Лукьяненко, Анна Витальевна; Sukovatyi, A.; Kuzmin, A.; Ryltseva, G.; Shishatskaya, E.
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    Biocompatible systems for controlled delivery of antiseptics for topical application / A. V. Vladimirova, A. V. Murueva, A. M. Shershneva [et al.] // J. Sib. Fed. Univ. Biol. - 2024. - Vol. 17, Is. 1. - P. 19-32 ; Журн. СФУ. Биол. - Cited References: 28 . - ISSN 1997-1389. - ISSN 2313-5530
   Перевод заглавия: Биосовместимые системы для контролируемой доставки антисептиков для местного применения
Кл.слова (ненормированные):
polyhydroxyalkanoates -- drug delivery system -- controlled delivery -- wound healing -- reconstructive technologies -- topical antiseptics -- полигидроксиалканоаты -- системы доставки лекарств -- контролируемая доставка -- заживление ран -- реконструктивные технологии -- антисептики местного действия
Аннотация: Controlled drug delivery is one of the frontier areas of science, which uses an interdisciplinary approach. The delivery systems offer numerous advantages over conventional dosage forms, such as improved efficacy and patient compatibility, reduced toxicity, and ease of use. Such systems often use micro- and nanoparticles as carriers for drugs, the prolonged effect of which is achieved due to the controlled slow release of the encapsulated drug. This study investigated the effects of encapsulation of various antiseptics (brilliant green, miramistin, and furacilin) and the chemical composition of the polymer on the yield, structure, size, drug release kinetics, and antibacterial activity of microparticles produced from resorbable polyesters of microbiological origin, polyhydroxyalkanoates. Microparticles with a 5.6–94.8 μm diameter were produced. The form of the active substance molecule has been found to be the most significant factor affecting the characteristics of polyhydroxyalkanoate microparticles. The surface structure of particles is rather determined by the chemical composition of the polymer, and the release kinetics to the model medium depends on the encapsulated drug. Microparticles based on PHAs loaded with brilliant green and furacilin showed antibacterial effects in S. aureus and E. coli cultures. The study demonstrated that microparticles with antiseptics encapsulated in them have potential as prolonged drug delivery systems and are of interest for further research.
Технология контролируемой доставки лекарств является одной из передовых областей науки, включающей междисциплинарный научный подход. Эти системы доставки обладают многочисленными преимуществами по сравнению с обычными лекарственными формами, такими как повышенная эффективность и биосовместимость, сниженная токсичность и простота использования. В таких системах в качестве носителей лекарственных средств часто используют микро- и наночастицы, пролонгированное действие которых достигается за счет контролируемого медленного высвобождения инкапсулированного лекарственного средства. В данном исследовании изучалось влияние включения различных антисептиков (бриллиантовый зелёный, мирамистин и фурацилин), а также химического состава полимера на выход, структуру, размер, кинетику высвобождения лекарственных средств и антибактериальную активность микрочастиц, полученных из резорбируемых полиэфиров микробиологического происхождения, полигидроксиалканоатов. Было получено семейство микрочастиц диаметром 5,6–94,8 мкм. Установлено, что наиболее значимым фактором, влияющим на характеристики микрочастиц полигидроксиалканоатов, является форма молекулы активного вещества. Структура поверхности частиц больше зависит от химического состава полимера, а кинетики выхода в модельную среду – от инкапсулированного лекарства. Микрочастицы на основе ПГА с депонированными бриллиантовым зеленым и фурацилином показали антибактериальное действие в культурах S. aureus и E. coli. Результаты продемонстрировали, что микрочастицы с депонированными антисептиками обладают потенциалом в качестве систем пролонгированной доставки и представляют интерес для дальнейших исследований.

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Держатели документа:
Siberian Federal University, Krasnoyarsk, Russian Federation
Institute of Biophysics SB RAS Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russian Federation
L.V. Kirensky Institute of Physics SB RAS Federal Research Center “Krasnoyarsk Science Center SB RAS” Krasnoyarsk, Russian Federation

Доп.точки доступа:
Vladimirova, A. V.; Murueva, A. V.; Shershneva, A. M.; Prudnikova, S. V.; Shabanov, A. V.; Шабанов, Александр Васильевич; Shishatskaya, E. I.

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Biodegradable polymer casting films for drug delivery and cell culture / A. V. Murueva, A. E. Dudaev, E. I. Shishatskaya [et al.] // Giant. - 2024. - Vol. 19. - Ст. 100314, DOI 10.1016/j.giant.2024.100314. - Cited References: 112. - The study was funded by State Assignment of the Ministry of Science and Higher Education of the Russian Federation (project No. FWES-2021–0025) . - ISSN 2666-5425
Кл.слова (ненормированные):
Degradable polyhydroxyalkanoates -- Copolymers P(3HB--3HV) and P(3HB--4HB) -- Solvent casting films -- Microstructure and properties -- Drugs -- Release kinetics -- Antibacterial activity -- Keratinocytes proliferation
Аннотация: In this work, for the first time, films were obtained based on biodegradable polyhydroxyalkanoates (PHAs) - a copolymer of 3-hydroxybutyrate-co-3-hydroxyvalerate P(3HB-co-3HV) and a copolymer of 3-hydroxybutyrate-co-4-hydroxybutyrate P(3HB-co-4HB), containing drugs of different chemical structure and action - antibiotics (ceftazidime, doripenem), antiseptic (chlorhexidine) and tissue regeneration stimulator (Actovegin) for drug delivery and cell culture. Using SEM, AFM and measuring the contact angles of water wetting, it is shown how PHAs composition and loading of films of P(3HB-co-3HV) and P(3HB-co-4HB) with drugs affect the porosity, roughness indicators and adhesive properties of the surface. The constructed films are a depot form of drug, the release of which in vitro is realized for a long time, without burst releases, corresponds to Korsmeyer-Peppas and Higuchi models with diffusion character. Films loaded with antibiotics have pronounced antibacterial activity and suppress the development of pathogens S. aureus and E. coli, without a pronounced negative effect on the adhesion and proliferation of epidermal cells. In the cultures of human keratinocytes HaCaT it was shown that the presence of actovegin, as well as ceftazidime in the films, exhibits a stimulating effect, increasing their number. The results demonstrate the suitability of the depot-films for cellular technologies and are promising for the reconstruction of tissues complicated by infection.

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Держатели документа:
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, Krasnoyarsk 660036, Russia
Siberian Federal University, 79 Svobodnyi av., Krasnoyarsk 660041, Russia
Infochemistry Scientific Centre, ITMO University, Lomonosova St. 9, St. Petersburg 191002, Russia
Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50 Akademgorodok, Krasnoyarsk, 660036, Russia
L.V. Kirensky Institute of Physics, Federal Research Center “Krasnoyarsk Science Center SB RAS, 50/12 Akademgorodok, Krasnoyarsk, 660036, Russia

Доп.точки доступа:
Murueva, A. V.; Dudaev, A. E.; Shishatskaya, E. I.; Ghorabe, F. D. E.; Nemtsev, I. V.; Немцев, Иван Васильевич; Lukyanenko, A. V.; Лукьяненко, Анна Витальевна; Volova, T. G.
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Biosynthesis and properties of sulfur-containing polyhydroxyalkanoates (PHAs) produced by wild-type strain Cupriavidus necator B-10646 / N. O. Zhila, K. Yu. Sapozhnikova, A. V. Berezovskaya [et al.] // Polymers. - 2023. - Vol. 15, Is. 4. - Ст. 1005, DOI 10.3390/polym15041005. - Cited References: 53. - The study was funded by State Assignment of the Ministry of Science and Higher Education of the Russian Federation (project No. 0287-2021-0025) . - ISSN 2073-4360
Кл.слова (ненормированные):
biosynthesis -- different precursors -- sulfur-containing PHAs -- chemical composition -- 3MP monomers contents -- properties
Аннотация: The study addresses the growth of the wild-type strain Cupriavidus necator B-10646 and the synthesis of sulfur-containing polyhydroxyalkanoates (PHA) by this strain on media containing fructose and three different precursors (3-mercaptopropionic acid, 3′,3′-dithiodipropionic acid and 3′,3′-thiodipropionic acid). By varying the concentration and number of doses of the precursors added into the bacterial culture, it was possible to find conditions that ensure the formation of 3-mercaptopropionate (3MP) monomers from the precursors and their incorporation into the C-chain of poly(3-hydroxybutyrate). A series of P(3HB-co-3MP) copolymer samples with different content of 3MP monomers (from 2.04 to 39.0 mol.%) were synthesized and the physicochemical properties were studied. The effect of 3MP monomers is manifested in a certain decrease in the molecular weight of the samples and an increase in polydispersity. Temperature changes are manifested in the appearance of two peaks in the melting region with different intervals regardless of the 3MP content. The studied P(3HB-co-3MP) samples, regardless of the content of 3MP monomers, are characterized by equalization of the ratio of the amorphous and crystalline phases and have a close degree of crystallinity with a minimum of 42%, = and a maximum of 54%.

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Держатели документа:
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, 660036 Krasnoyarsk, Russia
Basic Department of Biotechnology, School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi Av., 660041 Krasnoyarsk, Russia
Department of Medical Biology, School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi Av., 660041 Krasnoyarsk, Russia
L.V. Kirensky Institute of Physics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/38 Akademgorodok, 660036 Krasnoyarsk, Russia
Basic Department of Solid State Physics and Nanotechnology, School of Engineering Physics and Radio Electronics, Siberian Federal University, 26 Kirensky St., 660074 Krasnoyarsk, Russia
International and Interuniversity Centre for Nano Science and Nano Technology, Mahatma Gandhi University, Kottayam 686560, India

Доп.точки доступа:
Zhila, Natalia O.; Sapozhnikova, Kristina Yu.; Berezovskaya, Arina V.; Kiselev, Evgeniy G.; Shishatskaya, Ekaterina I.; Vasiliev, A. D.; Васильев, Александр Дмитриевич; Thomas, Sabu; Volova, Tatiana G.
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    Modification of polyhydroxyalkanoates polymer films surface of various compositions by laser processing / E. I. Shishatskaya, N. O. Zhila, A. E. Dudaev [et al.] // Polymers. - 2023. - Vol. 15, Is. 3. - Ст. 531, DOI 10.3390/polym15030531. - Cited References: 91. - The study was funded by the State Assignment of the Ministry of Science and Higher Education of the Russian Federation (Project No. 0287-2021-0025) . - ISSN 2073-4360
   Перевод заглавия: Модификация поверхности полимерных пленок полигидроксиалканоатов различного состава методом лазерной обработки
Кл.слова (ненормированные):
PHAs -- polymer films -- CO2 laser -- laser processing -- SEM -- AFM -- water contact angles -- fibroblast NIH 3T3 -- MTT test
Аннотация: The results of surface modification of solvent casting films made from polyhydroxyalkanoates (PHAs) of various compositions are presented: homopolymer poly-3-hydroxybutyrate P(3HB) and copolymers comprising various combinations of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), 4-hydroxybutyrate(4HB), and 3-hydroxyhexanoate (3HHx) monomers treated with a CO2 laser in continuous and quasi-pulsed radiation modes. The effects of PHAs film surface modification, depending on the composition and ratio of monomers according to the results of the study of SEM and AFM, contact angles of wetting with water, adhesion and growth of fibroblasts have been revealed for the laser radiation regime used. Under continuous irradiation with vector lines, melted regions in the form of grooves are formed on the surface of the films, in which most of the samples have increased values of the contact angle and a decrease in roughness. The quasi-pulse mode by the raster method causes the formation of holes without pronounced melted zones, the total area of which is lower by 20% compared to the area of melted grooves. The number of viable fibroblasts NIH 3T3 on the films after the quasi-pulse mode is 1.5–2.0 times higher compared to the continuous mode, and depends to a greater extent on the laser treatment mode than on the PHAs’ composition. The use of various modes of laser modification on the surface of PHAs with different compositions makes it possible to influence the morphology and properties of polymer films in a targeted manner. The results that have been obtained contribute to solving the critical issue of functional biodegradable polymeric materials.

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Держатели документа:
Department of Medical Biology, School of Fundamental Biology and Biotechnology, Siberian Federal University, Svobodnyi Av., 79, 660041 Krasnoyarsk, Russia
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, 660036 Krasnoyarsk, Russia
Chemistry Engineering Centre, ITMO University, Kronverkskiy Prospekt, 49A, 197101 Saint Petersburg, Russia
Basic Department of Biotechnology, School of Fundamental Biology and Biotechnology, Siberian Federal University, Svobodnyi Av., 79, 660041 Krasnoyarsk, Russia
L.V. Kirensky Institute of Physics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 660036 Krasnoyarsk, Russia
Federal Research Center “Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences”, 660036 Krasnoyarsk, Russia
Institute of Engineering Physics and Radio Electronics, Siberian Federal University, 660041 Krasnoyarsk, Russia

Доп.точки доступа:
Shishatskaya, Ekaterina I.; Zhila, Natalia O.; Dudaev, Alexey E.; Nemtsev, I. V.; Немцев, Иван Васильевич; Lukyanenko, A. V.; Лукьяненко, Анна Витальевна; Volova, Tatiana G.
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Biodegradable polyhydroxyalkanoates with a different set of valerate monomers: Chemical structure and physicochemical properties / T. G. Volova, N. O. Zhila, E. G. Kiselev [et al.] // Int. J. Mol. Sci. - 2023. - Vol. 24, Is. 18. - Ст. 14082, DOI 10.3390/ijms241814082. - Cited References: 99. - The study was funded by the Russian Science Foundation (project no. 23-64-10007) . - ISSN 1661-6596. - ISSN 1422-0067
Кл.слова (ненормированные):
copolymers -- P(3HB-co-3HV-co-4HV) -- P(3HB-co-3HV-co-3H4MV) -- physicochemical properties -- thermal behavior -- isothermal crystallization -- spherulites formation rate -- morphology
Аннотация: The properties, features of thermal behavior and crystallization of copolymers containing various types of valerate monomers were studied depending on the set and ratio of monomers. We synthesized and studied the properties of three-component copolymers containing unusual monomers 4-hydroxyvalerate (4HV) and 3-hydroxy-4-methylvalerate (3H4MV), in addition to the usual 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) monomers. The results showed that P(3HB-co-3HV-co-4HV) and P(3HB-co-3HV-co-3H4MV) terpolymers tended to increase thermal stability, especially for methylated samples, including an increase in the gap between melting point (Tmelt) and thermal degradation temperature (Tdegr), an increase in the melting point and glass transition temperature, as well as a lower degree of crystallinity (40–46%) compared with P(3HB-co-3HV) (58–66%). The copolymer crystallization kinetics depended on the set and ratio of monomers. For terpolymers during exothermic crystallization, higher rates of spherulite formation (Gmax) were registered, reaching, depending on the ratio of monomers, 1.6–2.0 µm/min, which was several times higher than the Gmax index (0.52 µm/min) for the P(3HB-co-3HV) copolymer. The revealed differences in the thermal properties and crystallization kinetics of terpolymers indicate that they are promising polymers for processing into high quality products from melts.

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Держатели документа:
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, Krasnoyarsk 660036, Russia
Basic Department of Biotechnology, School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi Av., Krasnoyarsk 660041, Russia
L.V. Kirensky Institute of Physics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/38 Akademgorodok, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Volova, Tatiana G.; Zhila, Natalia O.; Kiselev, Evgeniy G.; Sukovatyi, Aleksey G.; Lukyanenko, A. V.; Лукьяненко, Анна Витальевна; Shishatskaya, Ekaterina I.
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Biodegradable polyhydroxyalkanoates formed by 3- and 4-hydroxybutyrate monomers to produce nanomembranes suitable for drug delivery and cell culture / T. G. Volova, A. V. Demidenko, A. V. Murueva [et al.] // Technologies. - 2023. - Vol. 11, Is. 4. - Ст. 106, DOI 10.3390/technologies11040106. - Cited References: 116. - his research was funded by State Assignment of the Ministry of Science and Higher Education of the Russian Federation (project No. 0287-2021-0025) . - ISSN 2227-7080
Кл.слова (ненормированные):
degradable polyhydroxyalkanoates -- copolymers P(3HB-co-4HB) -- electrostatic molding -- membranes -- microstructure and properties -- drugs -- release kinetics -- antibacterial activity -- fibroblast proliferation
Аннотация: Biodegradable polyhydroxyalkanoates, biopolymers of microbiological origin, formed by 3- and 4-hydroxybutyrate monomers P(3HB-co-4HB), were used to obtain nanomembranes loaded with drugs as cell carriers by electrospinning. Resorbable non-woven membranes from P(3HB-co-4HB) loaded with ceftazidime, doripinem, and actovegin have been obtained. The loading of membranes with drugs differently affected the size of fibers and the structure of membranes, and in all cases increased the hydrophilicity of the surface. The release of drugs in vitro was gradual, which corresponded to the Higuchi and Korsmeyer-Peppas models. Antibiotic-loaded membranes showed antibacterial activity against S. aureus and E. coli, in which growth inhibition zones were 41.7 ± 1.1 and 38.6 ± 1.7 mm for ceftazidime and doripinem, respectively. The study of the biological activity of membranes in the NIH 3T3 mouse fibroblast culture based on the results of DAPI and FITC staining of cells, as well as the MTT test, did not reveal a negative effect despite the presence of antibiotics in them. Samples containing actovegin exhibit a stimulating effect on fibroblasts. Biodegradable polyhydroxyalkanoates formed by 3-hydroxybutyrate and 4-hydroxybutyrate monomers provide electrospinning non-woven membranes suitable for long-term delivery of drugs and cultivation of eukaryotic cells, and are promising for the treatment of wound defects complicated by infection.

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Держатели документа:
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, 660036 Krasnoyarsk, Russia
Institute of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi av., 660041 Krasnoyarsk, Russia
Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50 Akademgorodok, 660036 Krasnoyarsk, Russia
L.V. Kirensky Institute of Physics, 50/12 Akademgorodok, 660036 Krasnoyarsk, Russia
Chemistry Engineering Centre, ITMO University, Kronverkskiy Prospekt, 49A, 197101 Saint Petersburg, Russia

Доп.точки доступа:
Volova, Tatiana G.; Demidenko, Aleksey V.; Murueva, Anastasiya V.; Dudaev, Alexey E.; Nemtsev, I. V.; Немцев, Иван Васильевич; Shishatskaya, Ekaterina I.
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Production and properties of microbial polyhydroxyalkanoates synthesized from hydrolysates of Jerusalem artichoke tubers and vegetative biomass / T. G. Volova, E. G. Kiselev, A. V. Demidenko [et al.] // Polymers. - 2022. - Vol. 14, Is. 1. - Ст. 132, DOI 10.3390/polym14010132. - Cited References: 93. - This study was financially supported by Project "Agropreparations of the new generation: a strategy of construction and realization" (Agreement No. 075-15-2021-626) in accordance with Resolution No. 220 of the Government of the Russian Federation of 9 April 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning" (polymer synthesis, properties), and by the State Assignment of the Ministry of Science and Higher Education of the Russian Federation No. FSRZ-2020-0006 (films production, surface properties) . - ISSN 2073-4360

РУБ Polymer Science
Рубрики:
GLUCOSE-UTILIZING STRAIN
   RALSTONIA-EUTROPHA
   ASPERGILLUS-NIGER
   ACID
Кл.слова (ненормированные):
Jerusalem artichoke hydrolysates -- PHA synthesis -- productivity -- polyhydroxyalkanoates
Аннотация: One of the major challenges in PHA biotechnology is optimization of biotechnological processes of the entire synthesis, mainly by using new inexpensive carbon substrates. A promising substrate for PHA synthesis may be the sugars extracted from the Jerusalem artichoke. In the present study, hydrolysates of Jerusalem artichoke (JA) tubers and vegetative biomass were produced and used as carbon substrate for PHA synthesis. The hydrolysis procedure (the combination of aqueous extraction and acid hydrolysis, process temperature and duration) influenced the content of reducing substances (RS), monosaccharide contents, and the fructose/glucose ratio. All types of hydrolysates tested as substrates for cultivation of three strains—C. necator B-10646 and R. eutropha B 5786 and B 8562—were suitable for PHA synthesis, producing different biomass concentrations and polymer contents. The most productive process, conducted in 12-L fermenters, was achieved on hydrolysates of JA tubers (X = 66.9 g/L, 82% PHA) and vegetative biomass (55.1 g/L and 62% PHA) produced by aqueous extraction of sugars at 80 °C followed by acid hydrolysis at 60 °C, using the most productive strain, C. necator B-10646. The effects of JA hydrolysates on physicochemical properties of PHAs were studied for the first time. P(3HB) specimens synthesized from the JA hydrolysates, regardless of the source (tubers or vegetative biomass), hydrolysis conditions, and PHA producing strain employed, exhibited the 100–120 °C difference between the Tmelt and Tdegr, prevailing of the crystalline phase over the amorphous one (Cx between 69 and 75%), and variations in weight average molecular weight (409–480) kDa. Supplementation of the culture medium of C. necator B-10646 grown on JA hydrolysates with potassium valerate and ε-caprolactone resulted in the synthesis of P(3HB-co-3HV) and P(3HB-co-4HB) copolymers that had decreased degrees of crystallinity and molecular weights, which influenced the porosity and surface roughness of polymer films prepared from them. The study shows that JA hydrolysates used as carbon source enabled productive synthesis of PHAs, comparable to synthesis from pure sugars. The next step is to scale up PHA synthesis from JA hydrolysates and conduct the feasibility study. The present study contributes to the solution of the critical problem of PHA biotechnology—finding widely available and inexpensive substrates.

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Держатели документа:
Siberian Fed Univ, Sch Fundamental Biol & Biotechnol, Basic Dept Biotechnol, Krasnoyarsk 660041, Russia.
Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, Inst Biophys, SB RAS, Krasnoyarsk 660036, Russia.
Krasnoyarsk Sci Ctr SB RAS, LV Kirensky Phys Inst, Fed Res Ctr, SB RAS, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Fed Res Ctr, Krasnoyarsk Sci Ctr, Siberian Branch, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Volova, Tatiana G.; Kiselev, Evgeniy G.; Demidenko, Alexey V.; Zhila, Natalia O.; Nemtsev, I. V.; Немцев, Иван Васильевич; Lukyanenko, A. V.; Лукьяненко, Анна Витальевна; Project "Agropreparations of the new generation: a strategy of construction and realization" [075-15-2021-626]; State Assignment of the Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]
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Properties of degradable polyhydroxyalkanoates (PHAs) synthesized by a new strain, Cupriavidus necator IBP/SFU-1, from various carbon sources / N. O. Zhila, K. Yu. Sapozhnikova, E. G. Kiselev [et al.] // Polymers. - 2021. - Vol. 13, Is. 18. - Ст. 3142, DOI 10.3390/polym13183142. - Cited References: 78. - This work was financially supported by Project “Agropreparations of the new generation: a strategy of construction and realization” (Agreement No 075-15-2021-626) in accordance with Resolution No 220 of the Government of the Russian Federation of 9 April 2010, “On measures designed to attract leading scientists to the Russian institutions of higher learning” (strain isolation, polymer synthesis and investigation), and by the State Assignment of the Ministry of Science and Higher Education of the Russian Federation No. FSRZ-2020-0006 (study of film properties) . - ISSN 2073-4360
Кл.слова (ненормированные):
Cupriavidus necator IBP/SFU-1 -- cell growth and PHA synthesis -- various carbon sources -- PHA composition and properties -- polymer films
Аннотация: The bacterial strain isolated from soil was identified as Cupriavidus necator IBP/SFU-1 and investigated as a PHA producer. The strain was found to be able to grow and synthesize PHAs under autotrophic conditions and showed a broad organotrophic potential towards different carbon sources: sugars, glycerol, fatty acids, and plant oils. The highest cell concentrations (7–8 g/L) and PHA contents were produced from oleic acid (78%), fructose, glucose, and palm oil (over 80%). The type of the carbon source influenced the PHA chemical composition and properties: when grown on oleic acid, the strain synthesized the P(3HB-co-3HV) copolymer; on plant oils, the P(3HB-co-3HV-co-3HHx) terpolymer, and on the other substrates, the P(3HB) homopolymer. The type of the carbon source influenced molecular-weight properties of PHAs: P(3HB) synthesized under autotrophic growth conditions, from CO2, had the highest number-average (290 ± 15 kDa) and weight-average (850 ± 25 kDa) molecular weights and the lowest polydispersity (2.9 ± 0.2); polymers synthesized from organic carbon sources showed increased polydispersity and reduced molecular weight. The carbon source was not found to affect the degree of crystallinity and thermal properties of the PHAs. The type of the carbon source determined not only PHA composition and molecular weight but also surface microstructure and porosity of the polymer films. The new strain can be recommended as a promising P(3HB) producer from palm oil, oleic acid, and sugars (fructose and glucose) and as a producer of P(3HB-co-3HV) from oleic acid and P(3HB-co-3HV-co-3HHx) from palm oil.

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Держатели документа:
Basic Department of Biotechnology, School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi Av., Krasnoyarsk, 660041, Russian Federation
Federal Research Center, “Krasnoyarsk Science Center SB RAS”, Institute of Biophysics SB RAS, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Federal Research Center, “Krasnoyarsk Science Center SB RAS”, L.V. Kirensky Institute of Physics SB RAS, 50/38 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Federal Research Center, “Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences”, 50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Zhila, N. O.; Sapozhnikova, K. Yu.; Kiselev, E. G.; Vasiliev, A. D.; Васильев, Александр Дмитриевич; Nemtsev, I. V.; Немцев, Иван Васильевич; Shishatskaya, E. I.; Volova, T. G.
}
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10.

Properties of degradable polyhydroxyalkanoates with different monomer compositions / T. Volova, E. Kiselev, I. Nemtsev [et al.] // Int. J. Biol. Macromol. - 2021. - Vol. 182. - P. 98-114, DOI 10.1016/j.ijbiomac.2021.04.008. - Cited References: 109. - The part of the reported study on polymer synthesis and examination was funded by RFBR and KKRF [Grant No. 19-43-240012 “Biological and physical principles of production of new generation biomaterials”]. The work on production and investigation of polymer films was carried out as part of the State Assignment of the Ministry of Education and Science of the Russian Federation [Grant No. FSRZ-2020-0006] . - ISSN 0141-8130
Кл.слова (ненормированные):
Physicochemical properties -- Chemical composition -- Physicochemical properties -- Films -- Microstructure -- Surface properties
Аннотация: Purpose: To synthesize and investigate polyhydroxyalkanoates (PHAs) with different monomer composition and percentages and polymer films prepared from them. Results: Various PHAs: homopolymer poly-3-hydroxybutyrate P(3HB) and 2-, 3-, and 4-component copolymers comprising various combinations of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), 4-hydroxybutyrate (4HB), and 3-hydroxyhexanoate (3HHx) monomers were synthesized under specialized conditions. Relationships were found between the monomer composition of PHAs and their molecular-weight and thermal properties and degree of crystallinity. All copolymers had decreased weight average molecular weights, Mw (to 390–600 kDa), and increased values of polydispersity (3.2–4.6) compared to the P(3HB). PHA copolymers showed different thermal behavior: an insignificant decrease in Tmelt and the presence of the second peak in the melting region and changes in parameters of crystallization and glass transition. At the same time, they retained thermostability, and the difference between Tmelt and Tdegr was at least 100–120 °C. Incorporation of 4HB, 3HV, and 3HHx monomer units into the 3-hydroxybutyrate chain caused changes in the amorphous to crystalline ratio and decreased the degree of crystallinity (Cx) to 20–40%. According to the degree to which the monomers reduced crystallinity, they were ranked as follows: 4HB – 3HHx – 3HV. A unique set of films was produced; their surface properties and physical/mechanical properties were studied as dependent on PHA composition; monomers other than 3-hydroxybutyrate were found to enhance hydrophilicity, surface development, and elasticity of polymer films. Conclusion: An innovative set of PHA copolymers was synthesized and solution-cast films were prepared from them; the copolymers and films were investigated as dependent on polymer chemical composition. Results obtained in the present study contribute to the solution of a critical issue of producing degradable polymer materials.

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Держатели документа:
Siberian Federal University, 79 Svobodnyi av., Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
L.V. Kirensky Institute of Physics, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/12 Akademgorodok, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Volova, T.; Kiselev, E.; Nemtsev, I. V.; Немцев, Иван Васильевич; Lukyanenko, A. V.; Лукьяненко, Анна Витальевна; Sukovatyi, A.; Kuzmin, A.; Ryltseva, G.; Shishatskaya, E.
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